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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Cocultures of human colorectal tumor spheroids with immune cells reveal the therapeutic potential of MICA/B and NKG2A targeting for cancer treatment

Fig. 5

Anti-MICA/B induce immune-mediated anti-tumor effects through increased NK cell infiltration and activation in HT29 spheroids. HT29 spheroids were cocultured or not with CD19-CD14- PBMCs in the presence or not of anti-MICA/B antibodies or corresponding control isotype. We analyzed tumor cell death and spheroid destruction using (a, n = 10) microscopic pictures at 48 h, (b, n = 4) Incucyte live imaging or (c, n = 10) flow cytometry at 48 h, as well as (d and e) overall or (f and g) particular T and NK cells infiltration using flow cytometry (d, f and g, n = 10) and immunofluorescence (e, n = 1) at 24 h. We also analyzed by flow cytometry the expression of (h, n = 8) NKG2D, (i, n = 6) CD137, (j, n = 7) CD16 and (k, n = 8) NKG2A by NK cells in the IN and OUT compartments at 24 h. Statistical significance of immunofluorescence experiment was analyzed using the Mann-Whitney test, A panel using 2-way ANOVA and the other panels using the Wilcoxon matched-pairs signed rank test (* p < 0.05; ** p < 0.005, *** p < 0.001, **** p < 0.0001)

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