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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: HHLA2 in intrahepatic cholangiocarcinoma: an immune checkpoint with prognostic significance and wider expression compared with PD-L1

Fig. 3

Tumor infiltrating T cells, cytotoxic T cells (CTLs) and regulatory T cells (Tregs) and their correlation between HHLA2 and PD-L1 expression. Images of positive CD3 (a), CD8 (b) staining and the corresponding intra-tumor negative controls. Magnification × 50 for full views and × 400 for zoomed-in views (scale bar, 200 μm). Cells with double staining of CD4 and Foxp3 were identified as Tregs (Arrow) (c). Original magnification × 500 (scale bar, 50 μm). Scatter plot depicted the correlation between classic subsets of T cells and HHLA2 expression (d). High HHLA2 expression was significantly correlated with lower intra-tumor counts of T cells and cytotoxic T cells as well as increased ratio of Tregs to CTLs. PD-L1 expression was associated with higher intra-tumor counts of T cells and cytotoxic T cells (e and f). P -values were generated by Mann-Whitney U test. Error bars indicate median and interquartile range

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