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Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: HHLA2 in intrahepatic cholangiocarcinoma: an immune checkpoint with prognostic significance and wider expression compared with PD-L1

Fig. 4

CD68+ tumor associated macrophages (TAMs), CD163+ TAMs and CD20+ tumor infiltrating lymphocytes (TILs) and their correlation between HHLA2 and PD-L1 expression. Representative images of CD68 and CD163 staining (a; scale bar, 50 μm). No significant differences were found on the infiltrations of CD68+ TAMs, CD163+ TAMs as well as CD163+/CD68+ TAMs ratio between ICC with different HHLA2 expression levels (b). PD-L1 expression on TC was significantly correlated with a higher density of CD163+ TAMs and a higher CD163+/CD68+ TAMs ratio (c). PD-L1 expression on IC was significantly correlated with prominent infiltrations of CD68+ TAMs and CD163+ TAMs as well as a higher CD163+/CD68+ TAMs ratio (d). Images of positive CD20 staining and the corresponding intra-tumor negative control (e; scale bar, 50 μm). No significant differences were found on CD20+ TIL counts between ICC with different HHLA2 and PD-L1 expression levels (f). P -values were generated by Mann-Whitney U test. Error bars indicate median and interquartile range

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