Fig. 5
From: Collagen density regulates the activity of tumor-infiltrating T cells
T cells from different donors respond similarly to the surrounding collagen density. a Bar graph of normalized (Z-score) RNAseq read counts of a selected panel of differentially regulated genes. b-e Bar graphs of qRT-PCR analyses of the same panel of genes as in (a) in 3–4 different donors. Cultured cells were either PBMCs enriched for T cells (b), purified T cells (c), purified CD8+ cells (d) or TCR transduced T cells (e) cultured for 2 days in a high-density collagen matrix or a low-density collagen matrix. f Table of correlation analyses between COL1A1 gene and the same panel of genes as in (a) from a single-cell RNA sequencing dataset available in the Gene Expression Omnibus (GSE103322) (a-f) Asterisks indicate significantly regulated genes