Fig. 5
Transfer of TCL-loaded cDC1s enhances tumor-Ag-reactive T-cell presence in tumors and control established cancer progression. a Schematic representation of treatment and analysis for data shown in (b-n). Mice were intradermally (ID) injected with 106 B16-OVA cancer cells followed by ID injection of PBS control or 106 poly I:C and B16-OVA TCL-loaded cDC1s 5 days after, when tumors reached a size >10mm2 and < 55 mm2 (day 0). Either b-d tumor growth and survival were monitored or e-n animals sacrificed 3 days after cDC1 treatment for analysis of tumor and tumor-draining lymph node (tdLN). b Tumor growth and c survival curve. Combined data of 2 independent experiments with total n = 18 (Control) and n = 17 (cDC1s) mice are shown. *P < 0.05 by b Student’s t test on day 7 or c Mantel-Cox test. In a separate experiment, d tumors (n = 4) were dissected 9 days after cDC1 treatment and photographed. e-j Flow cytometric quantification of tdLN CD8+ T cells: e CD8+ CD44+ PD-1+, CD3+ CD8+ IFNγ+ or CD3+ CD4- IFNγ+ T cell number after re-stimulation with f OVA257–264 peptide or g B16-OVA TCL-loaded antigen-presenting cells (APCs) and tdLN CD4+ T cells: h CD4+ CD44+ PD-1+, CD3+ CD4+ IFNγ+ T cell number after re-stimulation with i OVA323–339 peptide-loaded APCs or j B16-OVA TCL-loaded APCs. k-n Flow cytometric quantification and representative histogram (gated on CD3+ and CD8+, CD4- or CD4+ cells) of tumor CD8+ T cells: CD3+ CD8+ IFNγ+ or CD3+ CD4- IFNγ+ T cell number after re-stimulation with k OVA257–264 peptide or l B16-OVA TCL-loaded APCs and tumor CD4+ T cells: CD3+ CD4+ IFNγ+ T cell number after re-stimulation with m OVA323–339 peptide-loaded APCs or n B16-OVA TCL-loaded APCs. Combined data of 2 independent experiments with total n = 12–17 (Control) and n = 11–15 (cDC1s) mice are shown. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t test