Fig. 7
Cell death-induced tumor Ag-loaded cDC1s are effective for treatment of largely anti-PD-1 refractory B16/F10 melanoma. a Schematic representation of treatment and analysis. Mice grafted intradermally (ID) with 5 × 105 B16/F10 cancer cells (day 0) were ID injected with PBS control (Control and αPD-1-treated groups) or 106 poly I:C and autologous B16/F10 TCL-loaded cDC1s (cDC1s- and cDC1s + αPD-1-treated groups) at day 6 & 13 as well as intraperitoneally injected with PBS control (Control and cDC1s-treated groups) or 100 μg anti-PD-1 antibody (αPD-1- and cDC1s + αPD-1-treated groups) at day 7, 10, 14 & 17 and tumor growth and survival monitored. b & d Tumor growth and c & e survival curve of mice treated as described in (a). The same control group (black) is presented in (b & d) as well as in (c & e), because data belong to the same experiment and are split for clarity. The graphs are displayed separately for better visibility. Combined data of 2 independent experiments with total n = 16 (Control, αPD-1 and cDC1s + αPD-1-treated groups) and n = 17 (cDC1s-treated group) mice are shown. *P < 0.05 by b & d Student’s t test at day 12 and 13 and **P < 0.01 by c & e Mantel-Cox test, ns: not significant