Fig. 3

CD11c-lineage-specific Dll1-ablated tumor-bearing mice exhibit no change in dendritic cells but increase CD11b⁺Gr1⁺ cell proportions. Myeloid populations were evaluated by flow cytometry on day 17–18 after LLC tumor initiation in Dll1 knockout and wild-type littermate mice. Percentage of indicated populations are shown in the tumor infiltrate (TIL) (a) and in a pool of splenocyte and LN cells (b). c Total cell yields in the splenocytes and tumor single cell suspensions. d Representative FACS plots for CD11b versus CD11c, Ly6C or Ly6G staining (c). Mean ± SEM, 5–7 mice per group; *, p < 0.05; **, p < 0.01