Fig. 6

STING agonist induces DC activation and maturation in vivo and in vitro. Tumors were harvested on day 19, digested and viable CD45⁺ immune cell subsets analyzed by flow cytometry. Co-stimulatory molecule CD86 expression on tumor-infiltrating MHC Class I expressing CD103⁺ (a) or CD11b⁺ (b) DC in non-treated controls (black lines or open bars) or DMXAA treated tumors (shaded gray histograms or black bars). Values are mean ± SD, n = 8–9 mice per group. ***, P ≤ 0.001; ****, P ≤ 0.0001. Cytokines (c), CC-family chemokines (d) or CXC-family chemokines (e) were measured in conditioned medium from bone marrow-derived DCs cultured 18 h in the presence (black bars) or absence (open bars) of 20 μg/mL DMXAA. Data are representative of two experiments. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; **** P ≤ 0.0001