Fig. 7From: STING agonist inflames the pancreatic cancer immune microenvironment and reduces tumor burden in mouse modelsPancreatic cancer epithelial cells are activated by STING agonist. a KPC1242 cells were stimulated with 10 μg/mL gemcitabine (GEM) alone, with GEM with 100 μg/mL DMXAA, DMXAA alone, 1 μg/mL lipopolysaccharide (LPS) as a positive control, or a vehicle negative control. Protein lysates were analyzed with antibodies to (a) phospho-STAT6 (pSTAT6), total STAT6 or GAPDH. b phospho-TBK1, total TBK1, or GAPDH, (c) phosphoIRF-3, total IRF-3 or GAPDH. Blots were probed with antibody against GAPDH as a loading control. Immunoblots were quantitated and represented graphically below each respective blot. d KPC1242 cells were incubated with GEM alone, GEM plus DMXAA, DMXAA alone, LPS alone, or vehicle control and apoptosis (d) and cell growth (e) measured. **, P ≤ 0.01; ****, P ≤ 0.0001. Values are mean ± SD, n = 4Back to article page