Fig. 2
From: Vaccination with nanoparticles combined with micro-adjuvants protects against cancer
The micron-sized MCT adjuvant displays depot effect when combined with CuMVTT-p33 nano-vaccine. a Confocal microscopy imaging of AF488 CuMVTT-p33 nano-vaccine following formulation with the micron-sized MCT adjuvant, 1) GFP signal of AF488 CuMVTT-p33 nano-vaccine 2) bright light field 3) an overlay 4–6 3D images with bright light field 4) an MCT crystal decorated with CuMVTT-p33 nano-vaccine particles. b A sketch illustrates the two prepared nano-vaccines, the 1st group consists of AF488 CuMVTT-p33 nano-vaccine and the 2nd consists of AF488 CuMVTT-p33 nano-vaccine formulated with MCT adjuvant. d Total number of AF488 CuMVTT-p33 nanoparticles in the popliteal LNs collected 3 h, 24 h, 48 h, 96 h and 216 h post-injection of the two prepared nano-vaccine groups in mice footpad. Statistical analysis by unpaired Student’s t test. c Confocal microscopy images of popliteal LNs 24 h and 216 h post-injection of the two prepared nano-vaccine groups in mice footpad, GFP signal was detected in LNs, whole mount view of z-stacks was acquired. One representative experiment of 3 similar experiments is shown