Fig. 6
From: Vaccination with nanoparticles combined with micro-adjuvants protects against cancer
The micron-sized MCT adjuvant shows comparable production of cytokines to B type CpGs and is superior to Alum in B16F10p33 melanoma. a Percentage of CD8+ IFN-γ + secreting cells (means ± SEM) in each vaccinated group. b Representative flow cytometry dot plots showing the frequency of CD8+ IFN-γ+ secreting cells in each vaccinated group. c Percentage of CD8+ TNF-⍺+ secreting cells (means ± SEM) in each vaccinated group. d Representative flow cytometry dot plots showing the frequency of CD8+ TNF-⍺+ secreting cells in each vaccinated group. e Percentage of dual IFN-γ+ and TNF-⍺+ secreting cells (means ± SEM) in each vaccinated tumour, gated on CD8+ cells in TILs. f Representative flow cytometry dot plots showing the frequency of dual IFN-γ+ and TNF-⍺+ secreting cells in each group, gated on CD8+ cells in TILs. Statistical analysis by Student’s t test. (4 mice per group), one representative of 3 similar experiments is shown