Fig. 5
From: STIM1 at the plasma membrane as a new target in progressive chronic lymphocytic leukemia
The pool of STIM1 in plasma membrane controls constitutive calcium entry (CE). a- Cytoplasmic (after permeabilization, total STIM1[t]) and plasma membrane staining of STIM1 (STIM1PM) on B-CLL cells from CE+ (n = 7) and CE- (n = 12) CLL patients. The isotype control is shown (black line). The mean fluorescence intensities (MFI) of representative CE- B-CLL cells are shown in the upper left corner of each plot in blue, and in the lower left corner in blue for CE+ B-CLL cells. b- Correlation between CE and tSTIM1T (upper panel) or STIM1PM (lower panel). c- Receiver operating curves (ROC) were generated to determine the area under the curve (AUC) and the optimal cut-off value to discriminate STIM1 high from STIM1 low patients. d- The effects of the anti-STIM1 mAb clone GOK on CE in CLL samples (n = 6). e- No effect of the anti-STIM1 mAb on anti-IgM Ca2+ response in CLL samples (n = 10). The r2 coefficient and P values are indicated when significant