Fig. 3

Immunostimulatory activity of ONC201. Immunohistochemical analysis of CD56+ (a) and granzyme B+ (b) cells in archival and post-ONC201 biopsy tumor tissue of an enzalutamide-refractory prostate cancer patient. Positive staining is depicted in gray color (P < 0.05). * denotes P < 0.05 by student’s two-tailed t test comparing the on-treatment tissue staining to archival tissue staining. c Heat map depicting maximum fold induction relative to baseline of immune cytokines and effector molecules in patients with PFS ≥ 12 weeks versus PFS < 12 weeks. * denotes P < 0.05 by student’s two-tailed t test comparing the on-treatment tissue staining to archival tissue staining. d Maximum fold change over baseline of immune cytokines and effector molecules in patients with PFS > =12 weeks by RECIST who received ONC201 at various dose levels once every three weeks or once weekly. Each error bar indicates SEM. * denotes P < 0.05 by student’s two-tailed t test comparing the maximum values to the baseline value. e PFS in patients who had at least a 50% induction in perforin following ONC201 once every three weeks or once weekly (P = 0.0078; HR 0.3211)