Fig. 3

HPV-specific T-cell responses and viral burden in patients treated with avelumab. a, HPV-specific responses in papilloma-infiltrating T-cells from patients 5 and 9 were measured by interferon gamma enzyme-linked immunospot assays. Antigen presenting cells were loaded with RNA encoding individual transcripts of HPV 6 (patient 5) or HPV 11 (Patient 9) genes. Patients 7 and 8 did not demonstrate HPV-specific responses in papilloma-infiltrating T-cells. b, HPV viral load for HPV 6 (patient 5) or HPV 11 (Patient 9) in papilloma and normal mucosa was determined by quantitative-polymerase chain reaction. c, The Tissue Inflammation Signature was calculated from NanoString IO 360 analysis for patients 5, 7, 8, and 9