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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Bispecific NKG2D-CD3 and NKG2D-CD16 fusion proteins for induction of NK and T cell reactivity against acute myeloid leukemia

Fig. 3

Modulation of effector cytotoxic capacity by BFP. NKG2D-CD3 and NKG2D-CD16 were immobilized to plastic as described in the methods section. Then PBMC of healthy donors were incubated on the immobilized fusion proteins or control. a Supernatants were analyzed for granzyme B and peforin levels after 24 h by Legendplex assays. Combined data obtained with 6 different PBMC donors are shown (Mean ± SEM). b, c Intracellular granzyme B and perforin expression was analyzed by flow cytometry. b Results obtained with untreated CD56+CD3 NK cells (n = 3 different donors, Mean ± SEM); (c) results obtained with CD4+ and CD8+ T cells cultured in the presence of BFP or control (identified by counterstaining for CD3, CD4 and CD8, (n = 4, Mean ± SEM) are shown

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