Fig. 3

Modulation of effector cytotoxic capacity by BFP. NKG2D-CD3 and NKG2D-CD16 were immobilized to plastic as described in the methods section. Then PBMC of healthy donors were incubated on the immobilized fusion proteins or control. a Supernatants were analyzed for granzyme B and peforin levels after 24 h by Legendplex assays. Combined data obtained with 6 different PBMC donors are shown (Mean ± SEM). b, c Intracellular granzyme B and perforin expression was analyzed by flow cytometry. b Results obtained with untreated CD56⁺CD3⁻ NK cells (n = 3 different donors, Mean ± SEM); (c) results obtained with CD4⁺ and CD8⁺ T cells cultured in the presence of BFP or control (identified by counterstaining for CD3, CD4 and CD8, (n = 4, Mean ± SEM) are shown