Fig. 1
From: Targeting of M2-like tumor-associated macrophages with a melittin-based pro-apoptotic peptide
Selective cytotoxicity of MEL-dKLA in M2-differentiated RAW264.7 macrophages. (a, b) Effects of dKLA, MEL, or MEL-dKLA on cell viability after incubation for 24 h. After incubation, cell viability was measured with MTS assays. The percent cell viability was standardized based on the absorbance of the PBS-treated control. *P < 0.05, **P < 0.01, ***P < 0.001 versus the dKLA group; #P < 0.05, ##P < 0.01, ###P < 0.001 versus the MEL group. (c) Cell cycle analysis revealed by propidium iodide (PI) staining. All results are expressed as means ± SEMs. (d, e) Annexin V-FITC and MitoTracker-Red CMXRos staining were measured by flow cytometry in (d) M1- and (e) M2-differentiated RAW264.7 macrophages. The cells were treated with PBS or 0.8 μM peptides. Each population of MitoTrackerlow Annexin V+ cells was normalized to the population treated with PBS as a control. *P < 0.05, **P < 0.01 versus the PBS group; ##P < 0.01 versus the dKLA group; $P < 0.05, $$P < 0.01 versus the MEL group. (n = 3)