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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Targeting of M2-like tumor-associated macrophages with a melittin-based pro-apoptotic peptide

Fig. 2

Induction of cell death due to mitochondrial membrane disruption. (a, b) Cellular respiration (a) and glycolysis (b) of peptide-treated cells were analyzed in real time with an XF24 flux analyzer. Oligomycin, FCCP, and Rot/AA were injected into XF24 plates sequentially after the baseline measurement. The y-axis shows the OCRs or ECARs, and the x-axis indicates the measurement number. (c–e) Basal respiration (c), ATP production (d), and maximal respiration (e) were compared between the experimental and PBS-treated control groups. (f-h) The cell energy phenotype, OCRs, and ECARs under basal and stressed conditions. All values are means ± SEMs (n = 3). *P < 0.05, **P < 0.01 versus the PBS group; #P < 0.05, versus the dKLA group; $P < 0.05 versus the MEL group (n = 3)

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