Fig. 5

Selective reduction of the M2-like TAM population by MEL and MEL-dKLA. (a) M1-like macrophages that infiltrated into the tumor stroma were stained as CD45⁺F4/80⁺CD86⁺ (upper panel) and M2-like TAMs were marked as CD45⁺F4/80⁺CD206⁺ (bottom panel). The cells are shown as dot plots within the F4/80 and CD86 or CD206 axis gated on CD45⁺ cells from total live-gated cells. (b, c) Data for each cell phenotype are displayed as the percentages of M1- and M2-like TAMs in CD45⁺ cells (left panel) and the exact number of M1- and M2-like TAMs counted per 10⁴ total single cells (right panel). (d) The M1/M2 ratio was calculated based on the percentage of F4/80⁺CD86⁺ cells and F4/80⁺CD206⁺ cells in CD45⁺ cells (left panel) or using the counted population in total sing cells (right panel). (e, f) The number of tissue-resident M1 or M2 macrophages were counted per 10⁴ total single cells. (g) CD4 T cells (CD45⁺CD4⁺CD8⁻), (h) regulatory T cells (CD4⁺CD25⁺Foxp3⁺), (i) CD8 T cells (CD45⁺CD4⁻CD8⁺), and (j) dendritic cells (CD45⁺CD11b⁺CD11c⁺) were detected within total live-gated cells. All values are presented as means ± SEMs (n = 5); *P < 0.05, **P < 0.01, ***P < 0.0001 versus the PBS group; #P < 0.05, ##P < 0.01 versus the dKLA group; and $P < 0.05 versus the MEL group (n = 5)