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Fig. 7 | Journal for ImmunoTherapy of Cancer

Fig. 7

From: Discovery of a novel natural killer cell line with distinct immunostimulatory and proliferative potential as an alternative platform for cancer immunotherapy

Fig. 7

Comparative analysis of expansion capacity of NK101 and NK-92. a NK101 and NK-92 cells were thawed from frozen vials and cultured in SCGM media supplemented with 20% FBS and 500 IU/ml of recombinant IL-2 for 32 days. Cells were subcultured every 2 days. Seeding and harvest cell density (lower dots and line, cells/ml) and viability (upper dots and line, %) are displayed together. Data represent mean ± SD of 3 independent experiments. b NK101 and NK-92 cells under stable growing conditions were seeded at a density of 2x105cells/ml and cultured for 20 days. Cells were harvested every two days and counted. Cumulative PDL was calculated by the formula described in Online Additional file 2. The numbers in the parenthesis indicates doubling time (Td). Data represent mean ± SD of duplicate wells from 2 independent experiments. c NK101 and NK-92 cells were stained with PE-conjugated anti-CD25, −CD122, and -CD132 antibodies, and analyzed by flow cytometry. Representative histogram plots from 3 independent experiments were displayed after gating singlets and live cells. Gray-shaded, dotted, and bold line indicates isotype control, NK-92, and NK101, respectively. The numbers in the histogram indicate mean fluorescent intensity. d NK101 or NK-92 cells were deprived of IL-2 for 24 h, and then treated with various concentration of IL-2 for 3 days. Expansion of cells was assessed by MTS assay, and the absorbance at 490 nm is normalized into 0 to 1 based on the minimum and maximum values for each cell line. Each dot represents mean ± SD of triplicate wells of two independent experiments

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