Fig. 3

Temporal-dependent phenotypic (72 and 144 h after the final NHS-muIL12 treatment) changes in myeloid/lymphoid cell profiles in the MB49^luc bladder TME of control Ig– (black bars) and NHS-muIL12–treated (grey bars) mice. Panels a-c represent differences in absolute number of cells/mg bladder weight of (a) Ly6C⁺ MDSCs, (b) Ly6G⁺ MDSCs and (c) in the frequencies of M1 of CD11b⁺Gr1⁻F4/80⁺ macrophages. (d) Total tumor TGF-β from control Ig– (black bars) and NHS-muIL12–treated (grey bars) mice at the 144-h time point (n = 5). e Frequencies of LAP⁺ CD3⁻ cells. Error bars represent mean + SEM, Student’s t-test; *P < 0.05, unless otherwise indicated, n = 3. Data are from a representative experiment that was repeated twice with similar results