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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Generation of monocyte-derived tumor-associated macrophages using tumor-conditioned media provides a novel method to study tumor-associated macrophages in vitro

Fig. 2

Characterization of in vitro generation methods of tumor-associated macrophages. (a) In vitro generated TAM were compared to classically and alternatively activated macrophages. Cells were stained on generation day 7 to assess surface expression of CD14, CD163, and CD206 analyzed by flow cytometry. Representative flow plots are shown. (b) The method used to generate TAM in vitro was validated using 6 conditions (TCM only, cytokines only, TCM + IL-4 + IL-10, TCM + IL-4 + M-CSF, TCM + IL-10 + M-CSF, TCM + IL- 4 + IL- 10 + M-CSF). Surface expression of CD14, CD163, and CD206 was measured on day 7 and analyzed by flow cytometry. Representative flow plots are shown

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