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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Generation of monocyte-derived tumor-associated macrophages using tumor-conditioned media provides a novel method to study tumor-associated macrophages in vitro

Fig. 3

Phenotype and functional characterization of in vitro generated TAM. (a) M2-like macrophages and in vitro generated TAM were flow stained for surface expression of CD14 and CD163/CD206 co-expression and were analyzed by real-time PCR for transcript expression of CD206. (b) M2-like macrophages and in vitro generated TAM were analyzed by real-time PCR for transcript expression of IL-6 (macrophage-associated inflammatory pathway), IL-10 (immunosuppressive cytokine), CCL2 (macrophage stimulating chemokine), c-Myc (pro-tumor oncogene), iNOS (inducible nitric oxide synthase; tumor-protecting [54]), and MMP9 (matrix metallopeptidase 9; mediated extracellular matrix degradation and release of bioactive VEGFα [55]). (c) Brightfield images of in vitro generated macrophages and TAM generated using TCM from different tumor types at day 7

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