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Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: Generation of monocyte-derived tumor-associated macrophages using tumor-conditioned media provides a novel method to study tumor-associated macrophages in vitro

Fig. 4

Human-derived TAM phenotype. (a) Melanoma and normal adjacent tissue were digested, stained for CX3CR1, CD163, and CD206 and analyzed by flow cytometry. Cell populations were first gated on the CD45+ and lineage (CD3, CD19, CD56) population. (b) SK-BR-3 in vitro generated TAMs were stained with antibodies for CX3CR1, CD163, and CD206 and analyzed by flow cytometry. Cell populations were first gated on the CD45+ and lineage (CD3, CD19, CD56) population

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