Fig. 1

OGD culture conditions impairs IFNy response and leads to abrogated tumor recognition by CD8 T cells. a and c Histogram plots of MHC-I expression of TC1 (a) and B16F10 (c) when cultured under different nutrient limiting conditions with our without stimulation of IFNy for 24 h. b and d Quantification of MHC-I expression of TC1 (b) and B16F10 (d) tumor cells of three independently performed experiments. Data is shown as normalized Mean Fluorescence Intensity (MFI) by dividing the MFI of the stained sample by the MFI of negative control. e and h B16F10 (e) or TC1 (h) tumor cells were cultured under normal or OGD conditions for 24 h with IFNy (white bars). OGD cultured tumor cells were cultured an additional 24 h under nutrient repleted conditions (gray bars). MHC-I surface expression of tumor cells was measured by flow cytometry. Quantitative data of three independent experiments is shown as normalized MFI. f and i Density plots of IFNy production of TC1 (f) or B16F10 (i) specific CD8 T cells upon tumor recognition measured by flow cytometry. MFI, mean fluorescence intensity. g and j Quantified data of (f and i). Data is shown as mean + − SD. Unpaired t test was used to calculate significance; n.s., not significant *, p < 0.05, **, p < 0.01, ***, p < 0.001