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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Metabolic stress in cancer cells induces immune escape through a PI3K-dependent blockade of IFNγ receptor signaling

Fig. 2

Gene transcription levels of IFNy-STAT1 relevant genes of tumor cells. Tumor cells were cultured under several nutrient limiting conditions for 24 h with or without IFNy. a and b Jak1 and Jak2 gene expression levels of TC1 (a) and B16F10 tumor cells (b) measured by qPCR. c and d Stat1 and Irf-1 gene expression levels of TC1 (c) and B16F10 tumor cells (d) measured by qPCR. e and f Tap1 and H2-Kb (MHC-I) gene expression levels of TC1 (e) and B16F10 tumor cells (f) measured by qPCR. Relative mRNA expression is shown compared to normal culture conditions without IFNy stimulation and normalized to UBC housekeeping gene expression. Representative data is shown as mean + − SD (n = 3). Unpaired t test was used to calculate significance; n.s., not significant *, p < 0.05, **, p < 0.01, ***, p < 0.001

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