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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Metabolic stress in cancer cells induces immune escape through a PI3K-dependent blockade of IFNγ receptor signaling

Fig. 3

Impaired phosphorylation of STAT1 responsible for impaired MHC-I upregulation. Tumor cells were cultured for 24 h under several nutrient limiting conditions with or without IFNy. a and b Western-blots of TC1 (a) and B16F10 tumor cells (b). Representative data out of three experiments is shown. c and e Quantification of western-blot results of total STAT1 protein expression of TC1 (c) and B16F10 (e). d, g and f, i Quantification of western-blot results of phosphorylated STAT1 at Y701 and S727 of TC1 (dg) and B16F10 (f, i). h and j Quantification of western-blot results of IRF-1 protein expression of TC1 (h) and B16F10 (j). Data was quantified by calculating signal ratio between protein of interest and beta-actin. Unpaired t test was used to calculate significance; n.s., not significant *, p < 0.05, **, p < 0.01, ***, p < 0.001

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