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Fig. 1 | Journal for ImmunoTherapy of Cancer

Fig. 1

From: PD-1 signaling affects cristae morphology and leads to mitochondrial dysfunction in human CD8+ T lymphocytes

Fig. 1

Characterization of gene expression profiles in CD8+ T cells after PD-1 ligation. a Representative dot plots showing CD25 and CD69 staining of primary human CD8+ T cells after 48 h stimulation with TCTRL, TACT, and TACT + PD1 beads. b Quantification of CD25- and CD69-expressing cells from dot plots as in a. Each dot represents a donor (n = 18). c Representative histograms showing IFNγ production by cells stimulated as in a for 24 and 48 h. d Quantification of data from c (n = 4). e Thymidine ([3H]-TdR) incorporation by cells stimulated as in a (n = 5). f PCA plot using the rlog-transformed values from the RNA-seq analysis. Each unique combination of cell stimulation and time is assigned a distinct color. g Hierarchical clustering dendrogram of the top 200 most-variable genes across samples. The heat map color code (left) uses the combination of cell stimulation/time as in f. h Venn diagrams showing the number of differentially expressed genes between indicated conditions at different times. For d and e, data show mean ± SEM. ** p < 0.01; *** p < 0.001, one-way (b) or two-way ANOVA (d, e) with Bonferroni post-test

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