Fig. 4
Validation of changes in expression of mitochondrial-related genes after PD-1 ligation. a STEM clusters of expression profiles in TACT and TACT + PD1 cells. Only significant profiles are shown, ordered by p value (bottom left). The line in each STEM cluster represents the average temporal expression profile for the genes assigned to the cluster. The number of genes in each profile is indicated (top right). b Scatter plot showing GO terms of STEM profiles A, E and F, represented as circles and clustered according to semantic similarities as determined by REViGO. Circle area is proportional to the significance of GO term overrepresentation; color indicates the log10 of the corrected p value for enrichment. c Time-course variation of the relative quantity (Rq) of indicated transcripts in TCTRL, TACT, and TACT + PD1 cells isolated from independent donors (n ≥ 3). d Representative immunoblots for proteins in CD8+ T cells stimulated as indicated (n ≥ 3 donors). e Densitometric analysis of immunoblots as in d. The Rq was calculated as the ratio between each protein and β-actin, taking the value for TCTRL cells as reference. For C and E, data are mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, using two-way ANOVA with Bonferroni post-test (c) or Kruskal-Wallis with Dunn’s post-hoc test for multiple comparisons (e); only significant differences are indicated