Fig. 7

PD-1 increases the formation of supercomplexes. a Representative blue native PAGE showing RCS formation in T_ACT, and T_{ACT + PD1}-stimulated cells (48 h). Blots were hybridized sequentially with anti-NDUFS3 (complex I), −Core2 (complex III) and -βF1-ATPase (complex V) antibodies. b Densitometric quantification of the blots shown in A (n = 4; *, p < 0.05, paired two-tailed Student’s t-test). c Blue native PAGE showing RCS formation in T_ACT and T_CTRL cells (48 h); hybridizations were as in a. d Densitometric quantification of the blots shown in c. Data shown are from a pool of three donors. e Relative MCJ/DnaJC15 mRNA levels in T_CTRL, T_ACT and T_{ACT + PD1} cells at different times post-stimulation with indicated beads. Values were normalized to unstimulated cells (time 0). Data are mean ± SEM (n = 3 independent donors). *** p < 0.001, two-way ANOVA with Bonferroni’s post-hoc test