Fig. 3

ttIL-12 induced a greater anti-metastatic effect than wtIL-12. Tumor-bearing mice were treated with control pDNA (pCtrl), wild-type IL-12 pDNA (pwtIL-12), or tumor-targeted IL-12 pDNA (pttIL-12). a Lungs were removed from 4 T1 tumor–bearing mice and were sectioned and stained with hematoxylin and eosin (H&E) to detect metastatic nodules. Representative sections are shown. Scale bars, 500 μm. b Livers and (c) lungs were removed from LM8 tumor–bearing mice, sectioned, and stained with H&E to detect metastatic nodules. Representative sections are shown. Scale bars, 500 μm for H&E and low-magnification immunohistochemistry. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001