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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Plasmacytoid dendritic cells orchestrate innate and adaptive anti-tumor immunity induced by oncolytic coxsackievirus A21

Fig. 2

CVA21 treatment induces cytokine-mediated bystander killing of CVA21-resistant cells. a. Conditioned-media (CM) was collected from healthy donor PBMC after 48 h (±CVA21 treatment; 1 pfu/PBMC) and analyzed for cytokines using a 48-plex multiplex assay. The mean fold change (n = 3) compared to untreated PBMCs is shown. Sample readings outside of the detection range were estimated using the assay range limits and are indicated by x. b. CVA21-resistant cell lines were cultured for 96 h in PBMC-CM (0, 0.1 and 1 pfu/cell CVA21 treatment for 48 h; n = 6 for KG-1, HL-60 and OPM2, n = 5 for kasumi-1) and cell viability was assessed using an MTS assay. Error bars indicate SEM. *denotes statistical significance

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