Skip to main content
Fig. 6 | Journal for ImmunoTherapy of Cancer

Fig. 6

From: Plasmacytoid dendritic cells orchestrate innate and adaptive anti-tumor immunity induced by oncolytic coxsackievirus A21

Fig. 6

Type I IFN and ICAM-1 are required for CVA21-induced anti-tumor immunity. a-d. HD-PBMC were treated with CVA21 for 24 h, with or without pre-treatment with type-1 IFN blockade or an ICAM-1-blocking antibody. NK cell CD69 expression (a) and NK cell CD107a/b degranulation (b) were measured in the presence of type I IFN blocking antibodies or corresponding isotype antibodies (n = 4). c. HD-PBMC were treated with CVA21, in the presence or absence of ICAM-1-blocking antibodies, and IFN-α secretion was examined by ELISA (n = 3). d. NK cell CD69 expression was determined following CVA21treatment, with or without pre-treatment with an ICAM-1-blocking antibody or isotype control (n = 3). e. ICAM-1 expression was measured on mature hematopoietic immune cells (CD45+) from primary AML patients (n = 14). f. Correlation of CVA21 response (death of AML blasts) with ICAM-1 expression on mature CD45+ hematopoietic cells from primary AML samples. Error bars indicate SEM. *denotes statistical significance

Back to article page