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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Type I interferon suppresses tumor growth through activating the STAT3-granzyme B pathway in tumor-infiltrating cytotoxic T lymphocytes

Fig. 5

IFN-I regulates expression of granzyme B in tumor-infiltrating and antigen-specific CTLs. a. RNA was isolated from tumor-infiltrating CTLs from MC38 (18 days after tumor injection) and MCA (96 days after MCA injection) tumor models as outlined in Fig. 2A and B and analyzed for gene expression using the Nanostring immunology gene panel. Genes whose expression levels are 2 or more folds different in tumor-infiltrating CTLs between WT and IFNAR1-KO mice were clustered and presented. Green color indicates higher in WT and red color indicates lower in WT mice. The numbers in the parentheses represent fold decrease in IFNAR-KO mice as compared to WT mice. b. Spleen cells from the WT and IFNAR1-KO mixed BM chimera mice were collected 14 days after boost and stained with MHCII-, CD8-, CD45.1-, CD45.2-specific mAbs and OVA tetramer. The activated (OVA tetramer-positive) WT (CD45.1+) and IFNAR1-KO (CD45.2+) CD8+ cells were gated as indicated and sorted for mRNA purification. c. RNAs were prepared from sorted cells as shown in B. Fifty ng RNA were analyzed for gene expression using the Nanostring immunology gene panel. Genes whose expression levels are 1.5 or more folds different between activated WT and IFNAR1-KO CD8+ T cells from the mixed chimera mice as shown in B were clustered and presented

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