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Fig. 7 | Journal for ImmunoTherapy of Cancer

Fig. 7

From: Type I interferon suppresses tumor growth through activating the STAT3-granzyme B pathway in tumor-infiltrating cytotoxic T lymphocytes

Fig. 7

IFNα and IFNβ-activated STAT3 binds to the Gzmb promoter in CTLs. a. Structures of the Gzmb promoter. The six putative ISRE sequences (right panel) and locations (left panel) are shown. b. Resting 2/20 CTLs were treated with recombinant IFNα and IFNβ protein, respectively, for 1 h. Nuclear extracts were prepared from these cells and analyzed for STAT3 activation using EMSA with the WT pSTAT3 consensus probe (Santa Cruz Cat# sc-2571) and mutant probe (Santa Cruz Cat# sc-2572). Black arrow points to the DNA-pSTAT3 complex. c. Nuclear extracts were prepared as in B and analyzed for STAT3 activation using EMSA with the Gzmb promoter DNA probe GP4 as indicated in A. To determine pSTAT3-DNA binding specificity, the WT pSTAT3 consensus probe as shown in B was used for cold probe competition at the indicated ratios relative to the GP4 probe as a specificity control. Black arrow points to the DNA-pSTA T3 complex

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