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Table 1 Hematology analysis of male and female mice of both groups at D15 and D49

From: Pre-clinical investigation of the synergy effect of interleukin-12 gene-electro-transfer during partially irreversible electropermeabilization against melanoma

Female mice

 

D15

D49

Group 1

Group 2

Group 1

Group 2

Parameters

Unity

Control

EGT

Control

EGT

White blood cells

10^3/μl

6.4 ± 1.7

2.9 ± 1.5

7.7 ± 2.1

6.3 ± 1.0

hemoglobin

g/dl

13.0 ± 0.3

10.9 ± 3.5

13.8 ± 1.1

14.0 ± 1.3

Red blood cells

10^6/μl

5.5 ± 3.2

6.7 ± 2.1

8.4 ± 0.8

8.4 ± 0.7

Hematocrit

%

26.6 ± 15.3

32.9 ± 11.6

40.6 ± 4.2

40.8 ± 3.9

Mean corpuscular volume

fl

48.7 ± 0.2

48.5 ± 2.6

48.3 ± 0.3

48.4 ± 0.7

Platelets

10^3/μl

333 ± 180

536 ± 297

376 ± 6

373.8 ± 62.5

Mean corpuscular hemoglobin

pg

28.3 ± 16.0

16.2 ± 1.5

16.4 ± 0.3

16.5 ± 0.3

Mean corpuscular hemoglobin concentration

g/dl

58.4 ± 32.7

33.6 ± 4.0

34.0 ± 0.7

34.2 ± 0.1

Male mice

 

D15

D49

  

Group 1

Group 2

Group 1

Group 2

Parameters

Unity

Control

EGT

Control

EGT

White blood cells

10^3/μl

5.6 ± 2.3

8.0 ± 1.9

8.4 ± 0.1

7.5 ± 3.4

hemoglobin

g/dl

13.0 ± 2.5

14.9 ± 1.0

13.9 ± 1.4

13.2 ± 0.7

Red blood cells

10^6/μl

7.6 ± 1.5

8.6 ± 0.6

8.3 ± 1.1

8.0 ± 0.3

Hematocrit

%

36.8 ± 7.3

42.5 ± 1.9

40.9 ± 3.5

38.7 ± 1.3

Mean corpuscular volume

fl

48.5 ± 0.1

49.6 ± 1.2

49.6 ± 2.6

48.3 ± 0.6

Platelets

10^3/μl

351 ± 6

345 ± 112

424 ± 62

368 ± 61

Mean corpuscular hemoglobin

pg

17.0 ± 0.1

17.3 ± 0.4

16.8 ± 0.6

16.4 ± 0.3

Mean corpuscular hemoglobin concentration

g/dl

35.2 ± 0.3

34.9 ± 1.1

34.0 ± 0.6

34.0 ± 1.1

  1. 2x25μg of pIL-12 were injected intradermally in the healthy skin of female and male C57Bl/6 mice. Electrotransfection was realized using HV-MV electrical parameters. Hematological parameters (white blood cells, hemoglobin, red blood cells, hematocrit, mean corpuscular volume, platelets, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration) were determined on 2 female and 2 male mice treated with control and 4 female and 3 male mice treated with GET sacrificed at D15 and on 2 female and 2 male mice treated with control and 4 female and 4 male mice treated with GET sacrificed at D49. Values represent mean ± SD