Fig. 6

Prime/boost therapy is dramatically improved via PD-1 blockade through reversal of tumor-specific T cell dysfunction. a Left: Intratumoral expression of PD-L1 assessed by Nanostring as described in Fig. 4 (n = 8). Right: Representative FACS data showing PD-1 expression on OVA-specific CD8⁺ T cells in the blood (PBL) or TME (TAL). b Day 12 IE9-mp1 tumor progression in mice following MIS416 Vax + MRB-OVA combined with IgG () or anti-PD-1 () (n = 8–9). c Compiled survival data of day 12 tumor-bearing mice following MIS416 Vax + MRB-CONT + anti-PD-1 (▲) or MIS416 Vax + MRB-OVA + IgG () or anti-PD-1 () (n = 4–19). d) CD3⁺ T cell infiltration was enumerated at either the tumor center or margins following MIS416 Vax + MRB-OVA + IgG or anti-PD-1. Adjacent pancreas was poorly infiltrated and served as an indicator of specific T cell trafficking to tumors. (n = 4). e Left Panel: Representative FACs plots depicting % OVA-specific CD8⁺ T cells by tetramer staining and corresponding IFN-γ production following ex vivo OVA_257–264 peptide stimulation in matched TME samples on day 25 following MIS416 + Vax + MRB-OVA + IgG or anti-PD-1 treatment. Right Panel: OVA-specific CD8⁺ TAL function was assessed based on ratio of % IFN-γ producing to tetramer⁺CD8⁺ T cells as shown (n = 4). Data presented as mean ± SEM. Data in b is from one representative experiment