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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: T cells expressing NKG2D chimeric antigen receptors efficiently eliminate glioblastoma and cancer stem cells

Fig. 2

NKG2D-BBz CAR-T cells lyse glioblastoma cells efficiently in vitro. a The schematic diagram of CD19-BBz and NKG2D-BBz vectors. b Expression of CARs were confirmed by FACS with F(ab)2 antibody for CD19-BBz and NKG2D antibody for NKG2D-BBz. c The cytotoxicity of NTD, CD19-BBz and NKG2D-BBz T cells against the indicated cell lines at different effector to target (E: T) ratios. The results are presented as the mean volume ± SD, * P < 0.05, *** P < 0.001. d The levels of cytokines, released by NTD, CD19-BBz and NKG2D-BBz T cells, were measured by ELISA after 16 h co-culture incubation at E:T ratio of 5:1. The results are presented as the mean volume ± SD, *** P < 0.001, ns, not significant. e, f The levels of perforin and granzyme B in the supernatant of the co-culture system described above were measured by ELISA. The results are presented as the mean volume ± SD, ** P < 0.01, *** P < 0.001

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