Fig. 5

NKG2D-BBz CAR-T cells homed to tumor sites. a The mice that received CAR-T cells treatment were sacrificed on day 7 and major organs were collected and immunohistochemical analysis was performed to detect the T cells using a CD3ζ antibody, scale bar = 100 μm. b B-NDG mice bearing U-251MG xenografts was sacrificed 7 days after CAR-T cell infusion and the tumor infiltrating lymphocytes (TIL) were analyzed by flow cytometry. The experiment was repeated three times independently. The results are presented as the mean volume ± SD, *** P < 0.001. c Tumors formed by U-251MG or U-87MG were dissected 7 days after NKG2D CAR-T cell infusion and immunohistochemical analysis was performed to detect the expression of GSC marker NESTIN, scale bar = 50 μm