Fig. 6

Safety of NKG2D-BBz CAR. a Six days after T cell infected by CAR lentivirus, a Click-iT EdU kit was used to detect DNA incorporation. This experiment was repeated three times independently. The results are presented as the mean volume ± SD; ns, not significant. b Three days after T cell infected by CAR lentivirus, 7-AAD staining was used to detect dead cells. The results are presented as the mean volume ± SD; ns, not significant. c Quantitative RT-PCR analysis for the expression of common oncogenes and tumor suppressor genes in the NTD, CD19-BBz and NKG2D-BBz T cells. The results are presented as the mean volume ± SD; ns, not significant. d Karyotype was performed to detect chromosomal alterations in NKG2D-BBz CAR transduction and control untransduced T cells