Fig. 1From: Tumor regression mediated by oncogene withdrawal or erlotinib stimulates infiltration of inflammatory immune cells in EGFR mutant lung tumorsThe immunosuppressive microenvironment in murine EGFRL858R –induced lung adenocarcinomas is partially reversed by erlotinib. (a) Experimental outline of tumor induction and erlotinib treatment. CCSP-rtTA; TetO-EGFRL858R mice and littermate controls on a doxycycline diet (green arrow) for 6–7 weeks were treated with erlotinib or left untreated for 2 weeks. Infiltrating immune cells were analyzed by flow cytometry. Quantification of (b) CD4 and CD8 T cells (c) FoxP3 positive CD4 T cells (d) Treg/ CD8+ T cell ratio and (e) PD-1 positive FoxP3- and FoxP3+ CD4 and CD8 T cells in the lungs (and spleens) of normal lung (NL) and tumor bearing CCSP-rtTA; TetO-EGFRL858R mice in the absence (−) and presence (+) of erlotinib for 2 weeks. Data are obtained from three independent experiments, (n = 4–6 mice per group). Data are shown as mean ± SD and * is P < 0.05 in a student’s t-testBack to article page