Fig. 2

Increased production and presence of immunostimulatory cytokines following erlotinib treatment. Quantification of the levels of indicated effector cytokines from (a) CD4 T cells and (b) CD8 T cells after PMA/ionomycin stimulation and intracellular cytokine staining of cells in the lungs of tumor bearing CCSP-rtTA; TetO-EGFR^L858R mice in the absence (−) and presence (+) of erlotinib for 2 weeks. Quantification of naïve and effector (c) CD4 and (d) CD8 T cells in lungs of CCSP-rtTA; TetO-EGFR^L858R tumor bearing mice untreated or treated with erlotinib for 2 weeks. Data are from three independent experiments, (n = 3 mice per group) (e) Quantification of chemokines and cytokines in lungs of tumor bearing CCSP-rtTA; TetO-EGFR^L858R mice in the absence (−) and presence (+) of erlotinib for 2 weeks. Proteins (from a panel of 23) with significantly different levels between untreated and erlotinib-treated lungs are shown. Data are shown as mean ± SD and * is P < 0.05 in a student’s t-test