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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Tumor regression mediated by oncogene withdrawal or erlotinib stimulates infiltration of inflammatory immune cells in EGFR mutant lung tumors

Fig. 3

Changes in T cells in the immune microenvironment are due to tumor regression. (a) Experimental outline of tumor induction and erlotinib treatment. CCSP-rtTA; TetO-EGFRL858R or CCSP-rtTA; TetO-EGFRL858R + T790M mice and littermate controls on a doxycycline diet (green arrow) were treated with erlotinib or left untreated for 2 weeks or taken off doxycycline diet. Infiltrating immune cells were analyzed by flow cytometry. Quantification of (b) CD4 and CD8 T cells, (c) FoxP3 positive CD4 T cells and (d) the Treg/ CD8 ratio in lungs of tumor bearing CCSP-rtTA; TetO-EGFRL858R or CCSP-rtTA; TetO-EGFRL858R + T790M mice in the absence (−) and presence (+) of erlotinib for 2 weeks or after doxycycline withdrawal. Data are from three independent experiments, (n = 4–6 mice per group). Data are shown as mean ± SD and * is P < 0.05 in a student’s t-test

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