Fig. 2

Immune cell infiltration in the TME. a Number of total NK cells/mm³ tumor. b Number of CD335+ (active) NK cells/mm³ tumor. c Number of non-Treg CD4 T cells/mm³ tumor. d Number of CD8 T cells/mm³ tumor. e Number of IFNg-secreting CD8 T cells/mm³ tumor. f Number of TRP2 antigen-specific CD8 T cells/mm³ tumor. g Correlation between the tumor volume and the number of TRP2 antigen-specific CD8 T cells in the TME. h Correlation between the number of active NK and CD8 T cells. R² was calculated by using a linear regression analysis. i Correlation between active NK and TRP2-specific CD8 T cells. The results in a, b, c, d, e, f and i are expressed as mean ± SEM (n = 4–6). a and b letters on the graphs indicate significantly different results when the superscript letters are different. The annotation “a,b” indicates no significant differences compared to a and b statistical groups. The presence of two different letters in two groups indicate a statistical difference (p < 0.05) between them; the same letter in two different groups indicates the absence of a statistical difference between these two groups