Oncolytic adenovirus drives specific immune response generated by a poly-epitope pDNA vaccine encoding melanoma neoantigens into the tumor site

Table 1 antigen nucleotide and peptide sequence and MHC specificity (reactive T cell subtype [12])

Antigen name	Reactive T cell subtype [12]	Nucleotide sequence in B16F1 cells	Peptide sequence
TRP2	CD8	AGCGTGTACGACTTCTTCGTGTGGCTG	SVYDFFVWL
Pbk	CD8	GACAGCGGCAGCCCTTTTCCTGCTGCCGTGATCCTGAGAGATGCCCTGCACATGGCTAGAGGCCTGAAGTACCTGCACCAA	DSGSPFPAAVILRDALHMARGLKYLHQ
Kif18b	CD4	CCTAGCAAGCCCAGCTTCCAAGAGTTCGTGGACTGGGAGAACGTGTCCCCTGAGCTGAACTCTACCGACCAGCCTTTCCTG	PSKPSFQEFVDWENVSPELNSTDQPFL
Cpsf3l	CD4	GAGTTCAAGCACATCAAGGCCTTCGACAGAACCTTCGCTGACAACCCCGGACCTATGGTTCGACCTTGGCAGTCTGCTAGC	EFKHIKAFDRTFADNPGPMVRPWQSAS Sequence in B16F10 cells [12]: EFKHIKAFDRTFANNPGPMVVFATPGM
Gp100	CD4	TGGAACAGACAGCTGTACCCCGAGTGGACCGAGGCCCAGAGACTGGAT	WNRQLYPEWTEAQRL

List of the antigens used in the study. Mutated amino acids of the peptide sequence compared to the wild type sequence are in bold and underlined in the table. Mutated amino acids of the gene Cpsf3l in the B16F10 cells compared to the wild type sequence are in bold and not underlined (the sequence in the table is the sequence found in B16F10 cells by Kreiter et al. [12])