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Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: Donor-derived cell-free DNA detects kidney transplant rejection during nivolumab treatment

Fig. 4

Phenotyping of the graft infiltrating lymphocytes isolated from the explanted kidney during nivolumab treatment. a Unstimulated graft infiltrating-lymphocytes were gated by size and granularity in the forward and side scatter. CD4+ and CD8+ T cells were gated within total CD3+ cells. b Intracellular IFN-γ, TNFα and IL-2 was determined in CD3+CD8 (representing the CD4+ population) and CD8+ T cells at 3 h of stimulation with PMA/ionomycin. c Presence of the presence of PD-1 in CD4+ and CD8+ T-cells was also determined. d Blockade of the PD-1 receptor by nivolumab was demonstrated by adding conjugated nivolumab to these cells and was compared with graft infiltrating lymphocytes of a rejected kidney from a patient who was not treated with nivolumab

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