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Fig. 1 | Journal for ImmunoTherapy of Cancer

Fig. 1

From: Combination therapy targeting both innate and adaptive immunity improves survival in a pre-clinical model of ovarian cancer

Fig. 1

Treatment with paclitaxel and carboplatin induces acute immunosuppression that is mediated by innate immune cells. Mice were inoculated orthotopically with ID8-Vegf-Defb29 ovarian cancer cells. Eight days later, the mice were injected with vehicle (Veh) or chemotherapy (Chemo). Two days later, peritoneal cells were harvested for analysis. a Volcano plots of gene expression data sets derived from FACS-sorted leukocytes (CD11b+ and CD3+). All probe sets are shown. The top differentially expressed genes in the myeloid population are named, and highlight coloring was applied to significantly differentially expressed (adj. p-value < 0.05) probe sets. The experiment was performed once with n = 3 biological replicates. b A heatmap of the top 35 upregulated genes after chemotherapy treatment in FACS-sorted CD11b+ cells. c Peritoneal cell suspensions were assessed by flow cytometry. Bar graphs show quantification of flow cytometry gating of CD3+ T cells, CD4+ T cells, and CD8+ T cells. d Flow cytometry gating of subsets of MHCII+ mature dendritic cells are shown as scatter plots and quantified at right. e Flow cytometry gating of subsets of F4/80+ macrophages are shown as scatter plots and quantified at right. Increased numbers of immunosuppressive ARG1+ IL-10+ myeloid cells are observed following chemotherapy. The experiment was performed twice with n = 4 biological replicates. Statistics were calculated using a two-sided unpaired t-test. Data are presented as mean ± SEM * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.0001

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