Fig. 1

The methylome of relatively high TMB lung cancer is unique, and many DMRs are recurrent. a The NOMs for every patient (represented by the x-axis). Red/green lines indicate the high/low TMB cutoff in our cohort; Bar plot (b) and MDS analysis (c) of all CpG sites; d Identification of differences in DNAm between high TMB and low TMB groups. e Scatter plot between methylation changes (delta-beta value, high TMB tumors vs. controls) and corresponding -log10(BH adj. P-value) for total assessed 865,918 sites was shown. CpG sites with deltabeta < 0.2 and -log10(BH adj. P-value) < 2 were defined as MVPs. The upper square indicates hypermethylated MVPs, and the bottom square indicates hypomethylated MVPs compared with controls; f The comparation of differently methylation sites and TMB of NSCLCs (LUAD/LUSC) in our cohort; g The comparation of differently methylation sites and TMB of NSCLCs (LUAD/LUSC) in TCGA dataset; h Consensus clustering of the DNA methylation reveals high and low TMB lung cancer groups of DNA methylation. 293 informative probes with strict screening parameters (s.d. > 0.2 between high and low TMB group, s.d. < 0.1 in high or low TMB group, |delta beta| > 0.2, BH adjusted P value < 0.05) were used for the consensus clustering