Fig. 4

Comparative analysis between Chinese and TCGA lung cancer populations. a The top 13 genes with most frequent mutations in our cohort with the decrease in NOMs; b Somatic interactions in our cohort. Such mutually exclusive or co-occurring set of genes can be detected using the somaticInteractions function in R/Bioconductor package ‘maftools’, which performs pair-wise fisher’s exact test to detect such significant pair of genes; c Comparison of TMB levels between smoking and non-smoking group. Unpaired t test P value =0.00015, Smoking group: mean = 218, Non-smoking group: mean = 101.40; d Heatmap plot to interpret the possible associations of mutation signature and TMB classification. Generally, high TMB patients with smoking history display a strong signal on signature 4 (the known signature associated with cigarette). Another high TMB group without smoking history display a dominant weight on signature 3 (The signature probably caused by failure of DNA double-strand break-repair in homologous recombination). Signature 3 and signature 12 occurred simultaneously in low TMB patients, regardless of smoking status; e The differential patterns of mutation between Chinese lung cancer population and TCGA LUAD/LUSC; f The comparation of Chinese LUAD/LUSC and TCGA LUSC/LUAD NOMs; g The relationship of TP53 and NOMs in Chinese NSCLSs and TCGA NSCLCs