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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Molecular and metabolic pathways mediating curative treatment of a non-Hodgkin B cell lymphoma by Sindbis viral vectors and anti-4-1BB monoclonal antibody

Fig. 2

SV alone and SV plus α4-1BB mAb stimulated cell cycle progression, cytokine production, and activation. a The numbers of significant differential (SD) expressed genes (upregulated and downregulated) of SV vs. untreated are as indicated. SD expressed genes were selected based on Deseq2 analysis (q < 0.05), |Log2FC| ≥ 1. b The enrichment scores for gene cluster of cell cycle for SV vs. untreated, SV+ α4-1BB vs. untreated and SV+ α4-1BB vs. SV respectively (“cell cycle” is the gene cluster with the highest enrichment score for these 3 comparisons). c The heat map representing SD expressed cytokine and chemokine genes (left, SV vs. untreated; right, SV+ α4-1BB vs.α4-1BB, Log2FC ≥ 1). Expression values are shown by Z-score. Genes are hierarchically clustered by one minus Pearson correlation. Red arrow, Ccl8, IL4, IL13 and IL21 expression. d The percentage of CD69+ T cells from all groups on day 2 after starting treatment was measured by flow cytometry. e GSEA enrichment plot of KEGG (SV + α4-1BB vs. untreated) TCR receptor signaling pathway. *, p < 0.05; **, p < 0.01, ***, p < 0.001

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