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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Molecular and metabolic pathways mediating curative treatment of a non-Hodgkin B cell lymphoma by Sindbis viral vectors and anti-4-1BB monoclonal antibody

Fig. 3

Sindbis virus plus α4-1BB combination induced higher cytotoxicity. a Splenocytes were mixed with fLuc-A20 lymphoma cells according to the ratio as indicated (splenocytes:lymphoma cells). Cytotoxicity corresponds to the reduction of normalized Luc activity (fLuc activity of A20 lymphoma cells only is normalized to 1). SV + tumor, α4-1BB + tumor, SV+ α4-1BB + tumor: tumor inoculated mice. SV, α4-1BB, SV+ α4-1BB: mice without tumor inoculation. b Splenocytes were harvested from all groups after 7 days treatment. The percentage of NKG2D+ cells was measured by flow cytometry (CD8 T cell gated). c The percentage of granzyme B+ and perforin+ cells was measured by flow cytometry (CD8 T cell gated). d Cytotoxicity associated genes were upregulated in SV + α4-1BB treated group. The heat map depicts the relative expression level of cytotoxicity associated genes. Expression values are shown by Z-score. Genes are hierarchically clustered by one minus Pearson correlation (day 7). Red square, granzyme b and perforin expression. Red arrow, Ifng and Stat4 expression. **, p < 0.01; ****,p < 0.0001

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